Coding

Part:BBa_K2477000:Design

Designed by: Jacob Premo   Group: iGEM17_UMaryland   (2017-10-25)


iGEM GFP (E0040) mutant with added PAM site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

Site directed mutagenesis was used to introduce a PAM site near the chromophore encoding region of GFP.


Source

The GFP gene was originally obtained from the iGEM 2017 DNA kit. It was then mutagenized.

References